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Biorad western blot protein ladder4/16/2024 No conjugate is needed for fluorescent blot detection. Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range These protein standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD and include three high-intensity reference.Molecular weight sizing of proteins after blot development.Monitoring blot transfer efficiency with easy orientation via multicolor standards.The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development. Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. LC-MS innovation: Improve analytical accuracy Which microbioreactor modules are best for you Pink bands can be visualized with a 532 nm laser or green LED.Which solid support is right for your oligo synthesis? Assemble the transfer sandwich and make sure no air bubbles are trapped in the sandwich. ![]() Place the gel in 1x transfer buffer for 1015 min. Transferring the protein from the gel to the membrane 1. For details, please refer to the Protein Blotting Guide, bulletin 2895.
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